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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 9;402(1):135–148. doi: 10.1016/j.virol.2010.03.009

Figure 5. Stimulation-dependent changes in coreceptor expression affect the percentage of CD4+ lymphocytes infected via each pathway.

Figure 5

CD4+ lymphocytes were infected with HIV-1 GFP pseudotype viruses after 3 days of CD3/CD28 costimulation or following an additional 10 days of culture with IL-2 to upregulate CCR5. Cells were incubated with coreceptor antagonists for 1 hour prior to and throughout the infection to define the pathway of entry. CCR5 and CXCR4 expression were analyzed by FACS on the day of infection, and four days later, infected cells were determined by FACS analysis for GFP expression. Data indicate the percentage of GFP+ cells infected through CCR5 (A) or CXCR4 (B) at day 3 and day 13 after isolation, and are representative of three independent experiments using cells from different donors. The percentage of cells expressing CCR5 (A) and CXCR4 (B) at the time of infection is indicated by an asterisk (*).