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. Author manuscript; available in PMC: 2011 Jun 20.
Published in final edited form as: Virology. 2010 Apr 9;402(1):135–148. doi: 10.1016/j.virol.2010.03.009

Figure 7. Efficiency of CCR5 use on Affinofile cells correlates with CCR5 use on CD4+ lymphocytes by R5X4 HIV-1.

Figure 7

Affinofile cells were induced to express a fixed level of CD4 (83,000 antibody binding sites (ABS)/cell) and varying levels of CCR5, then infected with HIV-1 luciferase pseudotypes in the presence of AMD3100 to block endogenous CXCR4. Infection was quantified 4 days later by luciferase activity in cell lysates. The results are shown as the luciferase levels at each CCR5 density normalized to infection levels on cells expressing the maximum density of CCR5 (A). The relationship between normalized infection of Affinofile cells expressing the lowest density of CCR5 and the proportion of total CD4+ lymphocyte infection that is mediated by CCR5 for each R5X4 Env pseudotype is also shown (B).