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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Periodontal Res. 2009 Nov 9;45(2):292–295. doi: 10.1111/j.1600-0765.2009.01241.x

Fig. 1.

Fig. 1

Purification of gelatinase-free human plasma fibronectin (FN) by gelatin affinity chromatography and sequential DMSO and urea elution. Diluted human plasma was centrifuged to eliminate aggregates and 500 μL sample was loaded on an analytical gelatin-Sepharose mini column (Vt 50 μL). Fractions representing loaded plasma (B), unbound protein (U), washes with chromatography buffer alone (W) or containing 1 M NaCl (N), equilibration with chromatography buffer (E), pre-elution with 3% DMSO (DMSO, 2-10 x Vt), and final elution with 4 M urea (Urea; 1-3) were analyzed by gelatin enzymography. This purification strategy produced gelatinase-free FN. Masses (Mr) and positions corresponding to FN and MMPs-2 and -9 are indicated