Table 3. Methylation of NRG1 exon 1 in invasive carcinomas (IC), morphologically normal tissue adjacent to IC and normal human breast tissues.
As control, normal tissue adjacent to benign lesions of the breast was used (control group). The methylation status of NRG1 is indicated as: U (unmethylated) or P (partially methylated: PCR products were obtained with both set of primers). M: methylated. ND: no determinate. The age of the patients at the moment that the sample was taken and type of lesion are indicated. IDC: invasive ductal carcinoma. ILC: invasive lobular carcinoma. DCIS: ductal carcinoma in situ. p
| Patient number | Age | Diagnostic | NRG1 |
|---|---|---|---|
| Group 1: Control Group (normal tissue adjacent to benign lesions) | |||
| 148115 | 60 | Bening breast disease | U |
| 135447 S8 | 77 | Apocrine metaplasia | U |
| 49101 | 56 | Fibroadenoma | ND |
| 136620 | 67 | Fibrocystic changes | P |
| 131665 | 64 | Fibrocystic changes | U |
| 85453 | 59 | Epithelial hyperplasia | U |
| 137340 S15 | 63 | Epithelial hyperplasia | P |
| 135990 S9 | 64 | Adenosis | U |
| 141007 S21 | 77 | Adenosis | U |
| 141300 S24 | 78 | Adenosis | U |
| S14 136880 | 59 | Papilloma | U |
| P=2/10=20% | |||
| Group 2: Breast tumors | |||
| 1004172 (287767) | 50 | IDC, DCIS (GRADE III) | P |
| 1003361 (285220) | 68 | IDC | P |
| 1004363 (276283) | 58 | IDC (HIGH GRADE) | P |
| 1004783 (214003) | 73 | IDC, DCIS(GRADE III) | P |
| 1003356 (261845) | 57 | IDC (GRADE II) | U |
| 1000851 (265718) | 59 | IDC, DCIS | P |
| 1004041 (286233) | 37 | IDC (GRADE III) | P |
| 1003341 (279135) | 44 | IDC, DCIS (HIGH GRADE) | P |
| 1004582 (296190) | 52 | IDC (GRADE III) | P |
| 1004092 (284610) | 78 | IDC (GRADE III) | U |
| 1000826 (279038) | 73 | IDC (GRADE III) | P |
| 1002833 (268295) | 64 | IDC (GRADE III) | P |
| 1001581 (230160) | 64 | IDC (GRADE III) | P |
| 1001380 (243278) | 59 | IDC (GRADE II) | U |
| 1001168 (254401) | 51 | IDC (GRADE II) | P |
| 1001484 (239086) | 76 | IDC (GRADE I) | P |
| 1003731 (241220) | 51 | IDC (GRADE III) | P |
| P= 14/17= 82.4% | |||
| Group 3: Morphologically normal tissue adjacent to IC | |||
| 140569 C26 | 67 | IDC | P |
| 145565 | 62 | IDC, DCIS | U |
| 145564 | 74 | IDC | P |
| 133360 | 57 | U | |
| 141008 S22 | 55 | IDC, DCIS | U |
| 145813 | 53 | IDC | P |
| 147043 | 73 | IDC, DCIS | M |
| 145563 | 65 | IDC | P |
| 139128 S19 | 84 | IDC | P |
| 141299 S23 | 75 | IDC, DCIS | P |
| 154855 | 59 | IDC | U |
| 143061 | 73 | IDC and ILC | P |
| 157678 | 92 | ILC | P |
| 156105 | 73 | ILC | U |
| P or M=9/14 = 64.3% | |||