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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Neuron. 2010 Apr 15;66(1):69–84. doi: 10.1016/j.neuron.2010.03.019

Figure 3. Pals1 deficiency depletes the neural progenitor pool by premature cell cycle exit.

Figure 3

(A) The Pals1 CKO cortex is smaller than littermate controls at E12. (B) At E14, both Pals1 Het and Pals1 CKO cortices are extremely small compared to controls. (C) A schematic of interkinetic movement of neural progenitors. (D) S-Phase cells transiently labeled by BrdU, and M-phase cells labeled with anti-P-H3 are substantially reduced in the Pals1 CKO brain. Intermediate reduction is seen in Pals1 Het embryo, compared to WT at E12 and E14 (D, E, F, respectively)(t-test; BrdU at E12, p<0.001; BrdU at E14, p<0.005; P-H3 at E12, p<0.01; P-H3 at E14, p<0.01; n=3 and n=4). (G, H) Neural progenitors exiting the cell cycle after 24 hours are double-labeled by BrdU and Ki67. Cells exiting the cell cycle are positive for BrdU, but negative for Ki67, and are increased in the Pals1 CKO versus controls. See also Figure S3.