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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Neuron. 2010 Apr 15;66(1):69–84. doi: 10.1016/j.neuron.2010.03.019

Figure 6. Pals1 loss disrupts apical complex proteins and adherens junctions.

Figure 6

(A) The expression of Pals1 in the neuroepithelium at E12 is diminished in a medial to lateral gradient in Pals1 CKO mutant mice versus WT. (B) At E12, the apical complex proteins aPKCλ and Crb2 were reduced in an analogous manner to the Pals1 loss in the Pals1 CKO. (C) Defects of Crb2 protein localization are observed as early as 9 hrs after Pals1 shRNA electroporation. The Crb2 protein diffuses in the cytoplasm in the shRNA treated cells, whereas the cells treated with control shRNA show apical localization. (D) Adherens junctions are relatively intact at E12 in Pals1 CKO mutant mice. At E13, adherens junctions are either displaced basally, or absent in Pals1 CKO mice. At E14, adherens junctions are completely absent in medial cortex of mutant mice. (E) Top panels: Scanning EM reveals fewer primary cilia in the Pals1 CKO neuroepithelium compared to wild type (arrowheads). Middle panels: Pericentrin staining was reduced in Pals1 CKO mice, indicating a reduction in basal bodies (the base of cilia). Lower panels: Pals1 CKO mice have shorter cilia than WT controls. In the Pals1 CKO, the apical membrane shows large and irregularly shaped particles compared to controls, which have smaller and relatively uniform particles at E12 (arrows). See also Figure S2.