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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3971–3979. doi: 10.4049/jimmunol.0901604

FIGURE 7.

FIGURE 7

Expression of FIZZ1 is inhibited in allergen-challenged Ndst1f/fTekCre+ mice. A, FIZZ1 expression in lung tissue of OVA-challenged Ndst1f/fTekCre+ mice and WT littermates as well as in unchallenged control mice was evaluated by immunohistochemistry using goat polyclonal Abs against murine FIZZ1. Representative images from each group at a magnification of ×20 are shown. B, Total FIZZ1 expression was quantitated from captured images (five fields per mouse) at ×10 magnification using ImageJ image analysis system and expressed as average FIZZ1-positive area per field. FIZZ1-positive type II alveolar epithelial cells in five randomly selected microscopic fields with no visible airways or blood vessels were counted (×40 magnification) and expressed as the average number of cells per field. A negligible number of FIZZ1-positive type II alveolar epithelial cells were present in saline-exposed control mice (n = 5 each for control and OVA-challenged WT littermates and 4 each for control and OVA-challenged Ndst1f/fTekCre+ mice). *, p < 0.05.