Figure 4.

SA-4-1BBL conjugate vaccine increases the intratumoral CD8⁺ Teff/Treg ratio. A, vaccination with SA-4-1BBL-E7 conjugates increased intratumoral CD8⁺ T cells and decreased CD4⁺Foxp3⁺ Treg cells, as determined by confocal microscopy, resulting in increased ratio of intratumoral CD8⁺ Teff/Treg cells (B; n = 4, * P < 0.05 vs. each other and naïve and SA-E7 controls). A minimum of 3 fields/tumor section were analyzed and pictures were taken under 20× objective.