Figure 2. A truncated profilaggrin is expressed in flaky tail mouse skin, which lacks the C-terminus.

Urea/Tris protein extracts from control wild type and ft/ft mice were fractionated by SDS/PAGE and immunoblotted with the peptide antibody developed to the C-terminus of mouse profilaggrin. (a) Coomassie brilliant blue-stained protein gel to control for loading, and (b), corresponding immunoblot probed with the C-terminal antibody. The antibody detects both full-length profilaggrin (P) and a putative C-terminal processing product (C) in wild type mouse extracts, however, these immunoreactive products are not detected in ft/ft mice (b). Note the lack of filaggrin (F) in ft/ft mice (a), observed previously¹¹. The mutant profilaggrin protein in ft/ft mice is faintly visible in (a) at an apparent molecular weight of ~215 kDa (*). This is readily detectable with a mouse filaggrin antibody as reported previously¹¹. Molecular weight marker sizes are shown at left.