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Orientation and direction dependence of the translocation parameters
| Pore direction | DNA orientation | ib (%) | σ (%) | τ (μs) |
|---|---|---|---|---|
| Forward | 3′ first | 10.6 | 2.3 | 115 ± 5 |
| Forward | 5′ first | 14.6 | 2.1 | 239 ± 5 |
| Backward | 3′ first | 12.1 | 2.4 | 110 ± 10 |
| Backward | 5′ first | 8.5 | 3.6 | 510 ± 40 |
Normalized blocked current and event duration for each subpopulation extracted from the Group-A clouds of the forward and backward experiments scatter plots.
