Table 2.

Top sequences from stage one with the highest approximate binding affinities (Run 2)

Sequence	Sequence selection rank	Approximate binding affinity rank	K∗	Position
				1	2	3	4	5	6	7	8	9	10	11	12	13
SQ027	40	1	3.38 × 10+01	W	C	V	W	Q	D	W	G	T	N	R	C	W
SQ100	159	2	8.45 × 10+00	W	C	V	W	Q	D	W	G	Q	T	Q	C	W
SQ087	214	3	4.26 × 10−02	N	C	V	W	Q	D	W	G	K	K	Q	C	W
SQ072	166	4	1.31 × 10−02	D	C	V	W	Q	D	W	G	Q	N	Q	C	W
SQ077	185	5	3.07 × 10−03	G	C	V	W	Q	D	W	G	G	N	Q	C	W
SQ040	102	6	4.48 × 10−04	Q	C	V	W	Q	D	W	G	T	N	Q	C	W
SQ098	238	7	3.46 × 10−04	K	C	V	W	Q	D	W	G	N	N	K	C	W
SQ017	50	8	6.78 × 10−05	N	C	V	W	Q	D	W	G	H	N	K	C	W
SQ025	69	9	4.27 × 10−05	N	C	V	W	Q	D	W	G	S	N	Q	C	W
SQ086	210	10	1.10 × 10−05	Q	C	V	W	Q	D	W	G	Q	N	Q	C	W
Variant E1	n/a	11	5.78 × 10−06	I	C	V	W	Q	D	W	G	A	H	R	C	T
SQ023	59	12	6.73 × 10−07	N	C	V	W	Q	D	W	G	E	N	Q	C	W
SQ024	65	13	4.35 × 10−07	S	C	V	W	Q	D	W	G	N	N	Q	C	W
SQ059	144	14	1.50 × 10−07	D	C	V	W	Q	D	W	G	T	N	K	C	W
SQ055	133	15	2.95 × 10−08	P	C	V	W	Q	D	W	G	N	K	Q	C	W
SQ046	112	16	2.06 × 10−08	P	C	V	W	Q	D	W	G	N	N	Q	C	W
SQ088	213	17	1.11 × 10−08	G	C	V	W	Q	D	W	G	K	N	Q	C	W
Native	n/a	18	4.27 × 10−12	I	C	V	V	Q	D	W	G	H	H	R	C	T

Mutations are indicated in boldface.