Fig. 4.

The direct examination of trajectories with prolonged residence within small regions (magnifying analysis) provided another estimate of the trapping area with radius , as well as estimates of the trapping time. (A) Radial standard deviation of the set of all localizations during a selected trajectory and localization accuracy of the magnifying analysis resulted in for N = 1,000; i.e., trapping occurs on the macromolecular scale (data shown: SM). (B) Trapping durations t_trap (N = 1,000) for PE, SM-COase and SM with exponential fits yielding characteristic decay times of 3 (PE), 3 (SM-COase), and 17 ms (SM). The histograms were normalized to the amplitudes of the exponential fit. Insets show two examples of sets of all localizations during a selected trajectory. (Scale bar, 100 nm.)