Table 1.
Results of single-molecule tracking of lipid diffusion
| D (μm2/s) | α | rtrap (nm) |
 (nm) |
 (ms) |
|
| SLB | 3.6 | 1 | - | - | - |
| PE | 0.4 | 0.7 | 11 | 3 | 3 |
| SM | 0.35 | 0.3 | 6 | 3 | 17 |
| SM+COase | 0.3 | 0.65 | 9 | 3 | 3 |
SLB: supported lipid bilayer; PE: Atto647N-phosphoethanolamine (live cell); SM: Atto647N-sphingomyelin (live cell); COase: SM diffusion after cholesterol depletion by COase treatment (live cell). D: free diffusion constant; α: time-fraction of free diffusion; rtrap: radius of trapping area determined by fitting P(Δr2,Δt) (N = 250); 
: radius of trapping area determined by the magnifying analysis (N = 1,000); 
: trapping time determined by the magnifying analysis (N = 1,000). Standard deviations of values are approximately 10–30%.