Fig. 1.

Repression of transcription by the Mja histones. (A) The relaxed pTerm plasmid template was incubated at 65 °C for 20 min with TBP, TFB, and RNAP, in the absence (Lanes 1, 8, 15, and 22), or with increasing concentrations of individual histones (specified above the panel and measured in terms of the monomer as specified in SI Text), and transcribed for 15 min at the same temperature. The transcript terminating at t_mcrA/M and the DNA recovery marker (RM) are shown. (B) residual transcription, data from A, quantified.