Fig. 6.

E6-AP knockout mice display elevated Ring1B level in various tissues and cerebellar Purkinje cells. (A) Brain, liver, spleen, heart, kidney, and lung tissues were homogenized in p300 buffer and analyzed following Western blotting for Ring1B expression as described under Materials and Methods. (B) Representative photomicrographs of immunohistochemical staining for Ring1B in the cerebellum (i and ii) and liver (iv and v), and for ubiquitinated histone H2A in the liver (vii and viii) of E6-AP wild type and knockout mice. In all sections, arrows point to representative stained nuclei of cerebellar Purkinje cells (i and ii) or hepatocytes (iv, v, vii, and viii); (iii, vi, and ix) represent semiquantitative assessment of the data presented in panels i and ii, iv and v, and vii and viii, respectively. For these assessments, the intensity of staining was scored by a pathologist that was blinded to the genotype, using an arbitrary scale of 0–3. Two-sided Student’s t test was used to assess statistical significance. Values are average ± SEM.