Fig. 1.
MyoG does not have a general role in actin-based functions. (A) Domain organization of several different MyTH/FERM myosins. The locations of the light-chain binding IQ motifs (lightly shaded ovals), single alpha-helix region (S), MyTH4 (M), and pleckstrin-homology domains (PH) are indicated. The MyoG heavy chain contains several regions enriched for the amino acids asparagine (N), serine (S), threonine (T), and glutamate (Q) and the location and major amino acids are indicated. (B) Confocal projections of vegetative control and myoG null mutants fixed and stained for actin. (Scale bar, 10 μm.) (C) Representative examples of the small-drop folate chemotaxis assay at 2 h. The well of either buffer or folate is toward the top of each panel. The black circle overlay indicates the circumference of the spot at the 0 h time-point. (Scale bar, 1 mm.) (D) Rates of growth and motility for control and myoG null mutants. Shown are the average doubling times (t1/2) of control and myoG null mutants in nutrient broth (axenic) or in B/r bacteria. Shown are the averaged results from two independent experiments (n ≥ 4). The speed (mean ± SD) of vegetative cells was determined by averaging the results of three independent experiments (total n ≥ 58 cells per strain).