Fig. 1.

Hrp48 is required for N expression and signaling in the developing wing. (A–H) Loss of Hrp48 reduces the expression of N target genes more significantly in females than in males. hrp48 LOF clones are marked by loss of GFP (A, C, E, and G) in wing discs (anterior to left, dorsal up). (A–D) Cut and Dpn expression is only partially lost in male discs (B and D, gray), whereas in females discs (E–H) it is abolished (F and H, gray). (I–L) Reduction of hrp48 results in a stronger reduction of N expression in females than in males. Knocking-down hrp48 expression using UAS-RNAi-hrp48 driven by dpp-Gal4 (marked by UAS-GFP, green) results in only a slight reduction in N protein (J) in male wing discs (I–J). (K–L) N protein (L) is clearly reduced in hrp48 knockdown female wing discs. (M–T) Hrp48 overexpression enhances N expression and signaling more significantly in females than in males. dpp-Gal4 driven hrp48 overexpression (M and Q, green) results in pronounced N up-regulation in females wing discs (R, gray) and no effect is observed in males (N, gray). dpp-Gal4 driven hrp48 overexpression (O and S, green) results in pronounced Dpn up-regulation in females wing discs (T, gray) while only a slight effect is observed in males (P, gray).