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. 2010 Apr 13;107(15):6888–6893. doi: 10.1073/pnas.0910941107

Fig. 4.

Fig. 4.

Plk4 is required for RhoA activation and Ect2 localization in cytokinesis. (A) Reduced levels of activated RhoA in Plk4+/− MEFs after release from nocodazole (mean ± SEM, n = 3, *P < 0.001 vs. heterozygous). (B) Representative images of MEFs in cytokinesis. Staining for Ect2 (red), α-tubulin (green), and DNA (blue) reveals Ect2 localization to the spindle midbody in Plk4+/+ but not Plk4+/− cells. (C) Reciprocal coimmunoprecipitation assay on HEK293T cells shows physical interaction of Flag-Plk4 with endogenous Ect2. (D) Ratio of GTP-bound/total RhoA in unsynchronized HEK293T cells transfected with 3Flag-Plk4, with or without prior depletion of Ect2 (siEct2), showing dependence of Plk4-induced RhoA activation on Ect2 (mean ± SEM, n = 2).