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. 2010 Mar 29;107(15):6912–6917. doi: 10.1073/pnas.0914930107

Fig. 2.

Fig. 2.

Mig-6 functions as a tumor suppressor. Reconstiting Mig-6 expression in (A) LN319 or (B) LN464 cells inhibits cell proliferation. Vec, empty vector control. (C) Knockdown of Mig-6 expression in U87 cells (Inset) accelerates cellular proliferation. shSC, hairpin control. (D) (Left) Reconstitution of Mig-6 expression in LN319 or LN464 cells attenuates anchorage-independent growth in soft agar. (Right) Histogram quantification. Error bars indicate ±SD [**, P = 0.002 (LN319) and 0.008 (LN464), n = 3]. (E) (Upper) Knockdown of Mig-6 expression in U87 cells promotes anchorage-independent growth in soft agar. (Lower) Histogram quantification. Error bars indicate ±SD (**, P = 0.001, n = 3). (F) Knockdown of Mig-6 expression in U87 cells promoted cell invasion in a Boyden chamber assay. (Upper) Representative images show the invasion of U87 cells expressing control (shSC and shMig-6-2) and targeting shRNA (shMig-6-1) after 16-h induction with 10% FBS followed by crystal violet (0.2%) staining. (Lower) Histogram quantification. Error bars indicate ±SD (**, P = 0.003, n = 3). (G) shRNA targeting Mig-6 has no effect on soft agar growth of LN319 cells. (H) Reconstitution of Mig-6 expression inhibits soft agar growth of U87 cells expressing Mig-6 targeting shRNA (shMig-6-1). Error bars indicate ±SD (*, P = 0.03; **, P = 0.01; n = 3).