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. 2010 Mar 29;107(15):6912–6917. doi: 10.1073/pnas.0914930107

Fig. 3.

Fig. 3.

Mig-6 suppresses EGFR signaling and promotes ligand-induced receptor degradation. (A) Knockdown of Mig-6 expression in U87 cells enhances the activation of EGFR and the downstream signaling pathway in response to EGF treatment. Cells were treated with EGF (20 ng/mL) for the indicated times and cell lysates were immunoblotted with the indicated antibodies. (B) Reconstitution of Mig-6 expression in LN319 cells attenuates the activation of EGFR and the downstream signaling pathway in response to EGF treatment. Cells were treated with EGF (20 ng/mL) for the indicated times and cell lysates were immunoblotted with the indicated antibodies. (C) Knockdown of Mig-6 expression in U87 cells delayed EGFR degradation induced by EGF stimulation. Cells were pretreated with cycloheximide (CHX) (10 μg/mL) for 1 h before being treated with EGF (20 ng/mL) in the presence of CHX for the indicated times and cell lysates were subjected to immunoblotting with the indicated antibodies. (D) Histogram quantification of EGFR level (normalized with actin level) in C. (E) Reconstitution of Mig-6 expression in LN319 cells promotes EGFR degradation induced by EGF stimulation. Cells were treated as described in C and lysates were subjected to immunoblotting with the indicated antibodies. (F) Histogram quantification of EGFR level (normalized with actin level) in E.