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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Signal. 2010 Mar 1;22(7):1054–1062. doi: 10.1016/j.cellsig.2010.02.008

Figure 7. Overexpression of ILK, but not inhibition of GSK3β, is sufficient for inducing upregulation of Hif-1α.

Figure 7

a. Cardiac myocyte were treated with various doses of Ad.ILK for 20 h or shRNA targeting GSK3β (Ad.GSK3βi) for 24 or 48 h, as indicated (n=2). b. Cardiac myocytes were treated with Ad.miR-199a-5p or a control virus for 16 h before applying Ad.ILK for an additional 20 h. At the conclusion of all the treatments listed above, protein was extracted and analyzed by Western blotting for the molecules indicated on the left of each panel (n=3). Hif-1α signal from experiments shown in a and b were quantified, normalized to GAPDH, averaged, and plotted relative to its control levels after adjusting it to 1. Error bars represent SEM and *p<0.001 vs. control.