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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Epigenomics. 2009 Oct;1(1):39–61. doi: 10.2217/epi.09.10

Figure 4. Pharmacologic reactivation in CLL cell lines.

Figure 4

The mRNA expression levels of eight genes were quantified using real-time RT-PCR in untreated WAC3CD5, MEC1 and MEC2 cell lines. These were also treated with the demethylating agent 5′-Aza, TSA or the combination of 5′-Aza and TSA. A housekeeping gene (HRPT1) was used as an internal control for all of the above genes, except for DLC-1 where GAPDH was used. The relative expression level of each gene was determined using 2-ΔΔCt, where Ct is the cycle threshold for positivity. 5′-Aza: 5-aza-2′-deoxycytidine; TSA: Trichostatin A.