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. Author manuscript; available in PMC: 2010 May 18.
Published in final edited form as: J Immunol. 2008 May 15;180(10):7019–7027. doi: 10.4049/jimmunol.180.10.7019

Figure 1. Flow cytometry analysis to demonstrate the expression of murine MHC molecules in CIITA-transfected DC-1 cells.

Figure 1

Flow cytometry data demonstrating the levels of H-2 Kb, H-2 Db, and I-Ab expression in CIITA-transfected cells and controls. The legend shows the vector plasmids with which the DCs were transfected. An immortalized dendritic cell line (DC-1) was transfected with CIITA or mutant CIITA (mtCIITA). Untransfected cells and cells transfected with the pcDNA3 vector backbone were used as a control. The expression of MHC I and II molecules was characterized using antibodies to MHC I H-2 Kb, H-2 Db, and MHC II I-Ab by flow cytometry analysis.