Table 1. Sizing the human interactome.

Average number of interactions detected per screen	Screening completeness of the repeat screen search space	Assay sensitivity	Estimated sampling sensitivity per repeat screen	Precision	Systematic false discovery rate	Stochastic false discovery rate	Size of the human interactome
Repeat screen experiment	Ensembl version 44.36f	hsPRS-v1 experiment	Repeat screen experiment	MAPPIT experiment	MAPPIT and repeat screen experiments	Repeat screen experiment	Combining all parameters
199	1.2%	17% ± 3.8%	53.1% ± 10%	79.4% ± 15.9%	13.6% ± 14.5%	11.7% ± 6.1%	130,111 ± 32,618 (73,548–199,688)

Estimation of the parameters of the binary interactome mapping framework. Average values ± 1 s.d. (standard deviation) of each parameter based on Monte Carlo simulation (n = 10,000 runs) are indicated after accounting for testing protein pairs in both bait-prey and prey-bait configurations.

^*The range of interactome size reported corresponds to the 95% confidence interval of the size predicted.