Figure 2.

Transcriptional changes during diauxic shift. During diauxic shift, mean RNA production decreases. This decrease is due to a decrease in burst size. The gene activation rate remains constant. (A) RNA expression profiles of a single-rDNA repeat at t=0, 90, 120, 180 min after the culture reaches OD₆₀₀=0.4 were determined using single-molecule FISH. Red lines are best fit from the burst model. Binsize is 20. (B–D) Mean RNA, normalized activation rates and burst sizes of the rDNA repeat at the different times. Error bars are standard errors from three independent experiments.