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. Author manuscript; available in PMC: 2010 May 18.
Published in final edited form as: Biochem J. 2009 Jul 15;421(3):397–404. doi: 10.1042/BJ20090528

Figure 4. Combined effects of ATPγS and polyubiquitin on peptidase activity of the 26S proteasome.

Figure 4

26S proteasome (0.7 nM) was assayed for peptidase activity using Suc-Leu-Leu-Val-Tyr-AMC as substrate. Panel A. Assays contained either no other additions (Con) or: ATPγS (400 μM); K48-linked polyubiquitin (510 nM), or; ATPγS (400 μM) and K48-linked polyubiquitin (510 nM). Panel B. Assays contained 20 μM ATPγS and indicated concentrations of K48-linked polyubiquitin. Panel C. Assays contained 100 nM K48-linked polyubiquitin and indicated concentrations of ATPγS. Data are expressed as mean values of triplicate assays (+/− SD). Similar results were obtain in three independent experiments for Panel A and two independent experiments for Panels B and C.