Figure 3. Absence of CCTα in cilia of CCTαG346E mutant cells correlates with abnormal cilia tips and disfunction of oral cilia.

(A to D) Phagocytosis capacity of CCTα-mutG346E strain was evaluated by exposition of cells to Indian ink. (A) Wildtype cell used as control; (B to D) CCTα-mutG346E cells exposed to Indian ink; (B) and (D) were unable to form food vacuoles and are void of black granules (n = 1448). (E to H) Tetrahymena wildtype (E) and CCTαG346E mutant (F to H) cells were processed for immunofluorescence using CCTα (affinity-purified) antibodies. Wildtype cells show CCTα localization in cilia while in cells carrying the CCTα-G346E mutation it is noticed the absence of CCTα signal in cilia. The images were taken with exactly the same settings of gain and contrast. Scale bar  = 10 µm.