Figure 1. The hypomorphic Gata1^low mutation increases the number of precursors and mature cDCs present in the spleen and decreases Gata1 expression in cDC precursors from the blood and in mature cDCs in the spleen.

A) Representative flow cytometrical analysis for CD11b/CD11c expression and frequency (B) and absolute number (C) of precursor and mature cDCs (B220^-/CD11b⁺/CD11c^low or CD11c^high, respectively) in blood, bone marrow and spleen from wild-type (blue bars) and Gata1^low (green bars) littermates. The B220 gating is presented in Supplementary Figure 1. Frequencies observed in 4-9 mice per group are presented as mean (±SD) of results. C) Absolute numbers were calculated by multiplying the frequency presented in B with the total cell number (Supplementary Table 1). D) Levels of Gata1 mRNA expressed by cDC populations (B220^-/CD11b⁺/CD11c^low or CD11c^high) prospectively isolated from blood, marrow and spleen, compared with the levels of Gata1 expressed by megakaryocytes (MK, CD41⁺/CD61⁺) and erythroid cells (Ery, CD119⁺/CD71⁺) purified from the marrow of the same animals. Expression levels are expressed as 2^-ΔCt and are presented as mean (±SD) of results obtained in 3 independent determinations per experimental point. Values statistically different by unpaired T-test analyses are indicated by * (p<0.05) and ** (p<0.01).