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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Exp Hematol. 2010 Mar 18;38(6):489–503.e1. doi: 10.1016/j.exphem.2010.03.006

Figure 3. Conventional dendrititc cell (cDC) precursors from the blood express reduced levels of a reporter gene driven by the regulatory sequences of Gata1 spared by the Gata1low mutation.

Figure 3

A) Organization of the Gata1 locus showing the position of the two promoters, the proximal (IE) and the distal (IT) promoter [36] and of two DNase hypersensitive sites, HS1 and HS2 [29-34]. The scissors mark the region deleted by the Gata1low mutation while the sequences -2.7 Kb upstream and 1.5 Kb downstream of the IE promoter that drive expression of the reporter (-2.7KbGata1GPF) are indicated with a line. B) Flow cytometrical determinations of the expression of the reporter gene by cDCs (B220-/CD11b+/CD11clow or CD11chigh, see Figure 1) from blood, bone marrow and spleen of -2.7kbGata1GFP∷Gata1+/y and C) -2.7kbGata1GFP∷Gata1low/y male littermates presented as contour-plots. The top panels present the levels of GFP expressed by cCDs from the marrow of wild-type and Gata1low mice not carrying the reporter gene.