Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Exp Hematol. 2010 Mar 18;38(6):489–503.e1. doi: 10.1016/j.exphem.2010.03.006

Figure 4. Conventional dendrititc cell (cDC) precursors present in the blood that express the GFP reporter express high levels of Gata1.

Figure 4

Levels of Gata1 mRNA expressed by cDCs (B220-/CD11b+/CD11clow, see Figure 1) prospectively isolated from the blood of -2.7kbGata1GFP∷Gata1+/y (left panels, red) and -2.7kbGata1GFP∷Gata1low/y (right panels, blue) mice, according to their levels of GFP expression. Negative controls are presented as gray histograms. The gating used for the purification and the reanalysis of the sorted cells for purity are presented in the top and middle panels, respectively. mRNA levels are expressed as 2-ΔCt. A representative experiment is presented.