Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Exp Hematol. 2010 Mar 18;38(6):489–503.e1. doi: 10.1016/j.exphem.2010.03.006

Figure 8. LPS induces AcH4 and reduces K27Me3H3 occupancy at the HS1 region of Gata1 in DCs expanded ex-vivo from wild-type mice.

Figure 8

ChIP was performed with anti-AcH4 and anti-K27Me3H3 antibodies using chromatin prepared from wild-type DCs expanded in the presence of GM-CSF and pulsed (bottom left panel) or not (top left panel) with LPS for 18hr. Controls for LPS stimulations were represented by ChIP analysis of the LPS-inducible GATA binding site of the Ifnγ promoter (top right panel) and flow cytometrical analysis for CD40 and CD86 surface expression (bottom right panels). AcH4 and K27Me3H3 binding is expressed as relative fold enrichment (RFE) after subtracting anti-GST antibody binding (negative control).