Fig. 1.

Effect of BPA on PC12 cell viability. (A) Cell viability of PC12 cells after 12, 24, 48, and 72 h treatments with/without several concentrations of BPA. Living cells were represented as a percentage of the total population of living and dead cells. (B) Photomicrographs of DAPI-stained PC12 cells treated for 24 h with several concentrations of BPA. Cells were stained with DAPI to visualize nuclear morphology. The percentage of apoptosis which presents a reduction in nuclear size, chromatin condensation, and nuclear fragmentation from two experiments was entered into a graph. (C) Photomicrographs of PC12 cells treated with the indicated materials for 5 days, respectively. PC12 cells were cultivated for 1 day in the condition of non-treated DMEM supplemented with Ham's F12 nutrient, 1% horse serum, 50 ng/ml NGF, 100 units/ml penicillin, and 100 µg/ml streptomycin, and were then cultured with/without several concentrations of BPA added to the medium. Control (Con), those cultured with/without BPA and NGF. ^*Significant difference from control (p < 0.05).