Fig. 3.

BPA-induced PC12 cell death was not mainly mediated by estrogen receptor. PC12 cells were treated with ER antagonist 30 min prior to treatment with BPA. (A) Viability of PC12 cell cultures was determined after treatment with compounds for 3 days. (B) Photomicrographs of PC12 cells cultured with BPA with/without ER antagonists added to DMEM supplemented with Ham's F12 nutrient, 5% fetal bovine serum, 10% horse serum, 50 ng/ml NGF, 100 units/ml penicillin, and 100 µg/ml streptomycin for 5 days. ^*Significant difference from control (p < 0.05).