Table 4.
Association between ARC-subCLST and pathobiological features
| Cluster | A1 | A2 | B | C1 | C2 | D |
|---|---|---|---|---|---|---|
| Stage I [191] | [36] | [42] | [23] | [41] | [25] | [20] |
| Size [mean ± SD mm] [24.8 ± 16.7 (7.0 to 120.0)] (Student's test, p < 0.0100: [A1,A2] vs [D]) | ||||||
| 19.3 ± 7.6 | 21.6 ± 13.8 | 26.4 ± 15.1 | 26.5 ± 19.2 | 26.7 ±17.7 | 35.2 ± 23.5 | |
| Pleural invasion (Fisher's exact test, p = 0.0002) | ||||||
| 0 [158] | 36 [100.0%] | 41 [97.6%] | 17 [74.0%] | 29 [70.7%] | 18 [72.0%] | 18 [72.0%] |
| 1[23] | 0 [0.0%] | 1 [2.4%] | 3 [13.0%] | 9 [22.0%] | 7 [28.0%] | 2 [22.0%] |
| 2 [10] | 0 [0.0%] | 0 [0.0%] | 3 [13.0%] | 3 [7.3%] | 0 [0.0%] | 4 [20.0%] |
| Lymphatic canal involvement (Fisher's exact test, p < 0.0001) | ||||||
| 0[61] | 12 [33.3%] | 31 [73.8%] | 8 [34.8%] | 6 [14.6%] | 2 [8.0%] | 2 [10.0%] |
| 1 [110] | 23 [63.9%] | 11 [26.2%] | 13 [56.5%] | 31 [75.6%] | 19 [76.0%] | 11 [55.0%] |
| 2 [18] | 1 [2.8%] | 0 [0.0%] | 2 [8.7%] | 4 [9.8%] | 4 [16.0%] | 5 [25.0%] |
| 3 [2] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 2 [10.0%] |
| Vascular involvement (Fisher's exact test, p < 0.0001) | ||||||
| 0 [129] | 29 [80.6%] | 41 [97.6%] | 13 [56.5%] | 28 [68.3%] | 10 [40.0%] | 6 [30.0%] |
| 1[46] | 6 [16.7%] | 0 [0.0%] | 8 [34.8%] | 12 [29.3%] | 11 [44.4%] | 7 [35.0%] |
| 2 [14] | 1 [2.7%] | 1 [2.4%] | 2 [8.7%] | 1 [2.4%] | 4 [16.0%] | 5 [25.0%] |
| 3 [2] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 2 [10.0%] |
| Grwoth activity [MIB1 index, mean ± SD %] [15.4 ± 17.5 (0.1 to 80.7)] (Student's t test, p < 0.0001: [A1,A2] vs [C2,D]) | ||||||
| 7.6 ± 12.7 | 4.4 ± 8.7 | 11.8 ± 13.0 | 15.1 ± 13.2 | 31.2 ± 21.1 | 33.5 ± 15.8 | |
| Stage IA [133] | [32] | [133] | [29] | [29] | [16] | [8] |
| Size [mean ± SD mm] [19.3 ± 7.6 (7.0 to 30.0)] (Student's t test, no significant difference) | ||||||
| 18.0 ± 6.7 | 15.7 ± 6.8 | 16.7 ± 7.7 | 18.0 ± 6.2 | 16.8 ± 4.9 | 14.8 ± 5.6 | |
| Pleural invasion (Fisher's exact test, p = 0.0188) | ||||||
| 0 [122] | 32 [100.0%] | 33 [100.0%] | 10 [83.3%] | 24 [82.8%] | 13 [81.3%] | 8 [100.0%] |
| 1[11] | 0 [0.0%] | 0 [0.0%] | 2 [16.7%] | 5 [17.2%] | 3 [18.7%] | 0 [0.0%] |
| Lymphatic canal involvement (Fisher's exact test, p < 0.0001) | ||||||
| 0[52] | 11 [34.4%] | 28 [84.8%] | 5 [36.4%] | 5 [17.2%] | 2 [12.5%] | 1 [12.5%] |
| 1[74] | 21 [65.6%] | 5 [15.2%] | 7 [63.6%] | 23 [79.4%] | 12 [75.0%] | 4 [50.0%] |
| 2 [6] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 1 [3.4%] | 2 [12.5%] | 2 [25.0%] |
| 3[1] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 1 [12.5%] |
| Vascular involvement (Fisher's exact test, not suitable for analysis) | ||||||
| 0[99] | 26 [81.3%] | 33 [100.0%] | 7 [58.3%] | 22 [75.9%] | 7 [43.8%] | 3 [37.5%] |
| 1[29] | 6 [18.7%] | 0 [0.0%] | 4 [33.3%] | 7 [24.1%] | 8 [50.0%] | 2 [25.0%] |
| 2 [5] | 0 [0.0%] | 0 [0.0%] | 1 [8.4%] | 0 [0.0%] | 1 [6.3%] | 3 [37.5%] |
| 3[0] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] |
| Growth activity [MIB1 index, mean ± SD %] [12.4 ± 15.4 (0 to 66.1)] (Student's t test, p < 0.0010: [A1,A2] vs [C2,D]) | ||||||
| 7.2 ± 13.4 | 4.4 ± 9.7 | 9.1 ±14.3 | 13.0 ± 12.1 | 26.3 ± 14.8 | 27.5 ± 13.6 | |
| Stage IB [58] | [4] | [9] | [11] | [12] | [9] | [12] |
| Size [mean ± SD mm] [43.2 ± 18.5 (10.0 to 120.0)] (Student's t test, no significant difference) | ||||||
| 30.0 ± 6.8 | 40.5 ± 13.7 | 35.1 ± 15.0 | 47.0 ±27.0 | 44.4 ± 18.5 | 48.9 ± 20.5 | |
| Pleural invasion (Fisher's exact test, p = 0.1715) | ||||||
| 0[34] | 4 [100.0%] | 5 [55.6%] | 8 [72.7%] | 5 [41.7%] | 5 [55.6%] | 6 [50.0%] |
| 1[15] | 0 [0.0%] | 4 [44.4%] | 1 [9.1%] | 4 [33.3%] | 4 [44.4%] | 2 [16.7%] |
| 2 [9] | 0 [0.0%] | 0 [0.0%] | 2 [18.2%] | 3 [25.0%] | 0 [0.0%] | 4 [33.3%] |
| Lymphatic canal involvement (Fisher's exact test, p = 0.5434) | ||||||
| 0[11] | 1 [25.0%] | 4 [44.4%] | 3 [27.3%] | 1 [8.3%] | 0 [0.0%] | 1 [8.3%] |
| 1[35] | 2 [50.0%] | 5 [55.6%] | 6 [54.5%] | 8 [66.7%] | 7 [77.8%] | 7 [58.4%] |
| 2 [11] | 1 [25.0%] | 0 [0.0%] | 2 [18.2%] | 3 [25.0%] | 2 [22.2%] | 3 [25.0%] |
| 3[1] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 1 [8.3%] |
| Vascular involvement (Fisher's exact test, p = 0.0547) | ||||||
| 0[31] | 3 [75.0%] | 9 [100.0%] | 6 [54.5%] | 6 [50.0%] | 3 [33.3%] | 3 [25.0%] |
| 1[17] | 0 [0.0%] | 0 [0.0%] | 4 [36.4%] | 5 [41.7%] | 3 [33.3%] | 5 [41.6%] |
| 2 [8] | 1 [25.0%] | 0 [0.0%] | 1 [9.1%] | 1 [8.3%] | 3 [33.3%] | 2 [16.7%] |
| 3 [2] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 0 [0.0%] | 2 [16.7%] |
| Growth activity [MIB1 index, mean ± SD %] [20.1 ± 26.0 (0.1 to 80.7)] (Student's t test, p < 0.0100: [A1,A2] vs [D]) | ||||||
| 10.8 ± 5.1 | 4.5 ±4.2 | 14.3 ± 11.7 | 20.0 ± 14.8 | 39.7 ± 28.2 | 37.5 ± 16.4 | |
ARC-subCLST, sub-clusters based on architectural element; SD, standard deviation; NA, not available.