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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Cancer Res. 2010 May 4;70(10):4081–4091. doi: 10.1158/0008-5472.CAN-09-3335

Figure-4. Levels of HPV16 early gene mRNA and protein in W12Ser4(EPI).

Figure-4

(A and B): Relative HPV16 early gene mRNA expression in W12Ser4(EPI), showing levels overall (A) and per viral copy, calculated using the formula [mean relative mRNA expression ratio ÷ mean relative HPV16 copy number] (B). (C): Western analysis of p53 and the HPV16 E6, E7, and E2 proteins. Beta-actin and TBP represent loading controls for total (T) and nuclear (N) protein extracts, respectively. For detection of E6/E7 protein, controls were total protein from Ncx/2 (negative) and W12Ser2p25 (positive). Since the p53 blot was generated by re-probing the E6 blot, the negative and positive lanes are reversed. For detection of E2 protein, controls were HeLa nuclear protein (negative) and full-length E2 purified from Sf21 insect cells (positive; a kind gift from Norman Maitland). (D): Viral protein quantification (arbitrary units) by densitometry. Error bars represent the standard error of technical replicate experiments. In (A), (B) and (D), the red arrowheads indicate the point at which invasiveness was acquired.