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. Author manuscript; available in PMC: 2010 May 19.
Published in final edited form as: Biochemistry. 2008 Dec 2;47(48):12787–12794. doi: 10.1021/bi801476j

TABLE 1. The efficiency of [125I]TID photoincorporation in amino acids within the δ subunit (cpm/pmol of PTH-derivative).

The 125I incorporation in each residue was calculated from the observed 125I release as described under “Experimental Procedures”, and the mass was calculated from the initial and repetitive yield.

Native Torpedo nAChR Purified Torpedo nAChR
Experiment I Experiment II Experiment III
Control Carb Carb Carb and PCP Control Carb
δM2-6 Ser262 < 0.3 0.6 1.7 <0.1 0.5 0.9
δM2-9 Leu265 43 1.4 2.5 5.2 17 0.7
δM2-13 Val269 130 2.4 3.3 3.9 44 1.2
δM2-18 Thr274 < 0.3 15 23 16 1.3 4.6
δ M2-M3 Ile288 1.0 6.5 11 6.4 0.8 0.8
δ M1 Phe232 < 0.5 5.0 8.3 4.4 1.0 1.6
Cys236 1.0 42 38 15 2.3 4.6
αM4 Cys412 7.7 13 38 41 100 80