TABLE 1. The efficiency of [125I]TID photoincorporation in amino acids within the δ subunit (cpm/pmol of PTH-derivative).
The 125I incorporation in each residue was calculated from the observed 125I release as described under “Experimental Procedures”, and the mass was calculated from the initial and repetitive yield.
Native Torpedo nAChR | Purified Torpedo nAChR | ||||||
---|---|---|---|---|---|---|---|
Experiment I | Experiment II | Experiment III | |||||
Control | Carb | Carb | Carb and PCP | Control | Carb | ||
δM2-6 | Ser262 | < 0.3 | 0.6 | 1.7 | <0.1 | 0.5 | 0.9 |
δM2-9 | Leu265 | 43 | 1.4 | 2.5 | 5.2 | 17 | 0.7 |
δM2-13 | Val269 | 130 | 2.4 | 3.3 | 3.9 | 44 | 1.2 |
δM2-18 | Thr274 | < 0.3 | 15 | 23 | 16 | 1.3 | 4.6 |
δ M2-M3 | Ile288 | 1.0 | 6.5 | 11 | 6.4 | 0.8 | 0.8 |
δ M1 | Phe232 | < 0.5 | 5.0 | 8.3 | 4.4 | 1.0 | 1.6 |
Cys236 | 1.0 | 42 | 38 | 15 | 2.3 | 4.6 | |
αM4 | Cys412 | 7.7 | 13 | 38 | 41 | 100 | 80 |