Table 1.
Primer | Nucleotide sequence | Amplicon size (bp) | Primer used in 25 μl RXN (pmol) | Annealing temperature (°C) | References |
---|---|---|---|---|---|
ompW (F) | GGTAGCCTTGGTGATATTGGTG | 208 | 12 | 59 | This study |
ompW (R) | TAGCAGCAAGTCCCCATGAGT | 12 | |||
ctxB (F) | GCCGGGTTGTGGGAATGCTCCAAG | 536 | 9 | 59 | Goel et al. (2007) |
ctxB (R) | CATGCGATTGCCGCAATTAGTATGGC | 9 | |||
rfbO1 (F) | TCTATGTGCTGCGATTGGTG | 638 | 10 | 59 | Goel et al. (2007) |
rfbO1 (R) | CCCCGAAAACCTAATGTGAG | 10 | |||
tcp-F | CGTTGGCGGTCAGTCTTG | 805 | 10 | 59 | Goel et al. (2007) |
tcp-R | CGGGCTTTCTTCTTGTTCG | 10 | |||
zot (F) | TCGCTTAACGATGGCGCGTTTT | 947 | 8 | 59 | Singh et al. (2001) |
zot (R) | AACCCCGTTTCACTTCTACCCA | 8 | |||
rtxC-F | CGACGAAGATCATTGACGAC | 265 | 10 | 59 | Chow et al. (2001) |
rtxC-R | CATCGTCGTTATGTGGTTGC | 10 | |||
ace-F | TAAGGATGTGCTTATGATGGACACCC | 309 | 10 | 59 | Kumar et al. (2009) |
ace-R | CGTGATGAATAAAGATACTCATAGG | 10 | |||
hlyA-F | GAGCCGGCATTCATCTGAAT | 480 | 10 | 59 | Kumar et al. (2009) |
hlyA-R | CTCAGCGGGCTAATACGGTTTA | 10 | |||
toxr-F | CCTTCGATCCCCTAAGCAATAC | 779 | 10 | 59 | Kumar et al. (2009) |
toxr-R: | AGGGTTAGCAACGATGCGTAAG | 10 | |||
ompU-F: | ACGCTGACGGAATCAACCAAAG | 869 | 10 | 59 | Kumar et al. (2009) |
ompU-R | GCGGAAGTTTGGCTTGAAGTAG | 10 | |||
ctx-F | GATACACATAATAGAATTAAGGATG | 461 | 10 | 59 | Olsvik et al. (1993) |
ctx-R | GGTTGCTTCTCA TCATCGAACCAC | 10 | |||
ERIC-F | ATGTAAGCTCCTGGGGATTCAC | Variable | 10 | 52 | Rivera et al. (1995) |
ERIC-R | AAGTAAGTGACTGGGTGAGCG | ||||
BOX A1R | CTACGGCAAGGCGACGCTGACG | Variable | 10 | 53 | Versalovic et al. (1994) |
qacEΔ1-F | ATCGCAATAGTTGGCGAAGT | 800 | 10 | 58 | Dalsgaard et al. (2000) |
Sul1-B | GCAAGGCGGAAACCCGCC | 10 | |||
int1-F | GCTGGATAGGTTAAGGGCGG | 592 | 10 | 55 | Hochhut et al. (2001) |
int1-R | CTCTATGGGCACTGTCCACATTG | 10 | |||
in-F | GGCATCCAAGCAGCAAGC | 10 | Collis and Hall (1992) | ||
aadA-R* | ATTGCCCAGTCGGCAGCG | 650 | 10 | 55 | Kazama et al. (1995) |
blaP1-R* | CTGGTTCATTTCAGATAGCG | 874 | 10 | 52 | Kazama et al. (1995) |
strA-F | TTGATGTGGTGTCCCGCAATGC | 383 | 10 | 57 | Hochhut et al. (2001) |
strA-R | CCAATCGCAGATAGAAGGCAA | 10 | |||
floR-F | TTATCTCCCTGTCGTTCCAGCG | 526 | 10 | 57 | Iwanaga et al. (2004) |
floR-R | CCTATGAGCACACGGGGAGC | 10 |
* Paired with in-F