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. 2010 May 17;189(4):671–679. doi: 10.1083/jcb.201001039

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© 2010 Lee et al.

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Figure 5. — Cortactin is required for parkin-CCCP–induced mitochondrial clearance. MEFs were transfected with control or cortactin RNAi and parkin-GFP, and incubated with or without CCCP for 16 h as indicated. (A) Cells were subjected to immunostaining with antibodies for Tom20 and parkin. Bar, 10 µm. (B) Cell lysates were subjected to immunoblotting with antibodies for Tom20, parkin, cortactin, and actin.