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. 2010 May 17;189(4):713–723. doi: 10.1083/jcb.200910142

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© 2010 Tien et al.

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Figure 4. — Ndc80 complex tracks with disassembling tips in the presence of Dam1 complex. (A) Representative two-color kymographs showing the tip tracking ability of 100 pM Ndc80 complex in the presence or absence of 500 pM Dam1 complex. Movement of GFP-tagged Ndc80 complex (green) is shown on disassembling microtubules (red). Concentrations are of free complexes in solution. (B) Mean tracking distance of Ndc80 complex per depolymerization event in the absence of Dam1 complex (n = 19) or in the presence of 500 pM Dam1 complex (n = 62). Error bars indicate SEM.