Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 17;189(4):713–723. doi: 10.1083/jcb.200910142

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Tien et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Ipl1 phosphorylation of the Dam1 complex regulates its interaction with the Ndc80 complex. (A) Representative kymographs showing changes in behavior of 10 pM Ndc80 complex with the addition of 500 pM S20A Dam1 complex with or without Ipl1 phosphorylation. Concentrations are of free complexes in solution. (B) Residence time distributions of 10 pM Ndc80 complex on microtubules without Dam1 complex (black histogram, n = 1,266 events), with 500 pM S20A Dam1 complex (green histogram, n = 1,081), and 500 pM Ipl1-phosphorylated S20A Dam1 complex (blue histogram, n = 974). Dotted lines show the weighted exponential fits used to determine dissociation rate constants, k_off. (C) Mean-squared displacement (MSD) is plotted against time for 10 pM Ndc80 complex on microtubules without Dam1 complex (black markers, n = 1,102), with 500 pM S20A Dam1 complex (green markers, n = 1,030), and with 500 pM Ipl1-phosphorylated S20A Dam1 complex (blue markers, n = 860). Dotted lines show the weighted linear fits used to determine diffusion constants, D. (D) Mean tracking distance of 100 pM Ndc80 complex per depolymerization event in the absence of Dam1 complex (n = 19), in the presence of 500 pM S20A Dam1 complex (n = 28), or in the presence of 500 pM Ipl1-phosphorylated S20A Dam1 complex (n = 39). Error bars indicate SEM.