Figure 4.

Induction of the β-catenin responsive protein cyclin D1 is enhanced in Nmp4 KO osteoblasts following 2 h r of OFSS compared to WT controls. (A) Representative immunoblot for cyclin D1 in osteoblasts from Nmp4 KO or WT osteoblasts maintained in static culture or subjected to OFSS for 2 hr. (B) Quantification of immunoblots illustrated in (A), n=3. The static vs. 2 hr OFSS experiment was repeated twice times with comparable results. *p<0.05 vs. static; #p<0.05 vs. WT flow.