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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Cell Physiol. 2010 May;223(2):435–441. doi: 10.1002/jcp.22057

Figure 5.

Figure 5

OFSS-induced reorganization of actin filaments into stress fibers, and formation of focal adhesions is qualitatively more extensive in Nmp4 KO osteoblasts compared to osteoblasts from WT mice. (A) Vinculin immunofluorescence to detect focal adhesions. (B) Texas-red-phalloidin labeling of F-actin. (C) Merged image of staining in (A) and (B). Cells were subjected to OFSS for 4 hr. (D) Immunoblot analysis to measure total levels of vinculin and actin in Nmp4 KO and WT osteoblasts maintained in static culture or subjected to 4 hr OFSS.