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. 2010 May;77(5):874–883. doi: 10.1124/mol.109.060301

Fig. 2.

Fig. 2.

Quantitative coimmunoprecipitation of AMPA subunits in hippocampal synaptosomal membranes 12 h after repeated morphine administration. AMPAR subunit composition was compared in hippocampal synaptosomal membranes from saline- and morphine-treated mice 12 h after discontinuation of morphine treatment. Immunoblots (IB) show the percentage of AMPAR subunits remaining (unbound fraction) after IP of synaptosomal membranes. Data shown represent the average of two independent experiments. The antibodies used for IB are indicated at the left. The left three lanes in each row show immunoblotting of IgG control immunoprecipitated sample. After IP with the respective antibody, the percentage remaining in the unbound fraction was calculated from the standard curve generated by controls of 100 (14 μg), 50 (7 μg), and 5% (0.7 μg) run on each blot. Results obtained indicate that morphine administration decreases the association between GluR1 and GluR2 and GluR3 and increases the association between GluR2 and GluR3.