Fig. 5.
Ethanol exposure for 4 h increases the association of PKCγ with α1-containing GABAA receptors in cultured cortical neurons. Receptors in the P2 fraction of control (lane 1) and ethanol-exposed cells (lane 2) were immunoprecipitated with α1 subunit antibody. Immunoprecipitated receptors were separated by SDS-PAGE and immunoblotted with PKCγ or PKCβ antibodies. The same blot was probed by α1 subunit antibody. PKCγ or PKCβ signal intensity was normalized to GABAA receptor α1 subunit signal intensity from the Western blots of the immunoprecipitate. A, association of PKCγ with α1-containing GABAA receptors was increased 92.88 ± 23.60% in cultured neurons after 4 h ethanol exposure. B, there was no change in PKCβ association with α1-containing receptors after 4-h ethanol exposure. Data represent mean ± S.E.M. *, p < 0.05, Student's t test.