Fig. 7.
Ethanol increases PKCγ, but not PKCβ or PKCε, colocalization with GABAA receptor α1 subunit in cultured cortical neurons. Cell were exposed to ethanol for 4 h and stained with anti-α1 and PKC antibodies. Immunostaining of α1- and PKC-labeled cells were examined under confocal microscope. The total number of α1/PKC colocalized cells were normalized to total number of α1-stained cells on the same coverslip and presented as percentage control values. Ethanol exposure for 4 h increased PKCγ association with GABAA receptor α1 subunit by 70.17 ± 15% (A). In contrast, ethanol exposure did not alter the association of PKCβ or PKCε with GABAA receptor α1 subunit compared with controls (B and C). Data represent mean ± S.E.M. *, p < 0.05, Student's t test.