Fig. 8.
PKCγ RNA interference transfection inhibits ethanol-induced internalization of GABAA receptor α1 subunit in cultured cortical cells. Cerebral cortical neurons were transfected with scrambled or PKCγ siRNA. The P2 fraction was analyzed by SDS-PAGE analysis and probed with PKCγ, -β, and -ε antibodies. A, PKCγ-specific, but not scrambled, siRNA decreased the expression of PKCγ by 76.09 ± 1.087%, whereas the expression of PKCβ and -ε were not altered. B, selective PKCγ siRNA inhibits ethanol (50 mM)-induced internalization of GABAA receptor α1 subunit. *, p < 0.05 compared with vehicle, Newman-Keuls test.