PXR activation in hAPP mice restores P-glycoprotein expression and transport
of NBD-CSA. A, P-glycoprotein (P-gp) Western blot of brain capillary
membranes isolated from vehicle-treated wild-type and hAPP mice and hAPP
mice dosed with 25 mg/kg PCN once a day for 7 days. β-Actin was
used as protein loading control (pooled tissue from 20 mice per group). B,
accumulation of NBD-CSA in brain capillaries from vehicle-treated wild-type
and hAPP mice and PCN-treated hAPP mice. Capillaries were incubated with 2
μM NBD-CSA for 1 h alone or with PSC833. C, data after capillary
image analysis. Data are mean ± S.E.M. for 10 capillaries from
one preparation (pooled tissue from 20 mice per group). Shown are arbitrary
fluorescence units (scale 0–255). ***, significantly lower than
control, P < 0.001.