Skip to main content
. 2010 May;77(5):828–835. doi: 10.1124/mol.109.061507

Fig. 6.

Fig. 6.

siRNA-Mediated knockdown of TAO2 and TAK1 and pharmacological inhibition of TAK1 enhance As2O3-induced growth suppression of CFU-L colony formation from AML patients. A, peripheral blood mononuclear cells from two AML patients were transfected with control siRNA or TAO2 siRNA and were subsequently incubated in methylcellulose in the absence or presence of As2O3 (0.5 μM). CFU-L colony formation was assessed, and data are expressed as means ± S.E. of the percentage of colony formation of samples treated with control siRNA only. B, as in A, but using TAK1-specific siRNA. C, peripheral blood mononuclear cells from three AML patients were plated in a methylcellulose assay system with As2O3 (0.5 μM), in the absence or presence of 5Z-7-oxozeaenol (100 nM), as indicated. CFU-L colony formation was assessed and data are expressed as means ± S.E. of the percentage of colony formation of untreated samples. Paired t test analysis comparing the effects of As2O3 in the absence or presence of 5Z-7-oxozeaenol showed a paired p value of 0.01009. UT, untreated.