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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Matern Fetal Neonatal Med. 2010 May;23(5):409–416. doi: 10.1080/14767050903168424

Figure 2.

Figure 2

HPLC elution profiles of the standard compounds of bupropion and its metabolites.

Figure 2A: HPLC elution profile of the standard compounds of bupropion and its metabolites as detected by UV at 254 nm. (1): hydroxybupropion (6.7 minutes), (2): erythrohydrobupropion (7.7 minutes), (3): threohydropbupropion (8.5 minutes), (4): bupropion (10.9 minutes).

Figure 2B: HPLC elution profile of standard custom-synthesized [3H]-bupropion as detected by β-RAM-flow-through on-line detector.

Figure 2C-F: Chromatograms of perfusion medium and tissue homogenate after 240 minutes of perfusion as detected by β-RAM flow-through on-line detector.

(C): Maternal perfusate in absence of placenta.

(D): Maternal perfusate in presence of placenta.

(E): Fetal perfusate in presence of placenta.

(F): Placental tissue homogenate.

Two major peaks at 8.5 minutes and 10.9 minutes are detected in the maternal and fetal perfusate and placental tissue, corresponding to the metabolite (3) and parent drug (4).