Following gut ischemia/reperfusion (IR), the proinflammatory transcription factor, AP-1, is increased, as is the downstream, proinflammatory enzyme iNOS. Both are associated with gut inflammation, mucosal injury, and dysfunction. When the enteral nutrient, arginine, is added to the hypoperfused gut, iNOS expression and AP-1 DNA-binding activity are further increased. Arginine is a known precursor to iNOS, but the mechanism by which arginine increases AP-1 is unknown. There is a recently described, NO-mediated protein kinase G pathway that may explain the increase in AP-1 by arginine. PPARγ is believed to inhibit the proinflammatory pathway at the level of AP-1, protecting the hypoperfused gut. Glutamine increases PPARγ DNA-binding activity and may be a novel PPARγ agonist. When PPAR γ is activated, it heterodimerizes with the retinoic acid receptor (RXR) and then binds to the peroxisome proliferator response element (PPRE), resulting in target gene expression.